Comparative in Vitro Study of Thrombolytic Effect of Punica Granatum and Azadirachta Indica

The aim of the study was to investigate the thrombolytic activity of Punica granatum and Azadirachta indica over canine, bovine, caprine and avian blood clot using streptokinase and water as positive and negative control respectively. Using an in vitro thrombolytic model, the clot lysis activity of A. indica was observed as 48.06±0.59%, 50.01±1%, 47.6±0.69% and 28.03±0.35% and of P. granatum as 44.73±0.65%, 48.53±0.56%, 43.83±0.6% and 47.17±0.32% in cattle, dog, goat, poultry respectively. The percentage (%) clot lysis was statistically significant (p<0.05) when compared with vehicle control whereas streptokinase showed clot lysis 43.13±0.9%, 58.4±0.72%, 55.07±0.58% and 44.67±0.65% respectively. We observed that these herbal extracts possess thrombolytic properties that could lyse blood clots in vitro. However, in vivo clot dissolving properties and active component(s) of these extracts for clot lysis are observed to be protein in nature as the protein extract of Azadirachta indica exhibits different protein bands between 16.6 to 90.4 kda where as that of Punica granatum exhibits only 2 bands of 10.9 and 17.3 kDa MW. So the individual components from these bands can be used for further research.